Identifikasi Senyawa Kelompok Antosianin dalam Ekstrak Bunga Telang (Clitoria ternatea) dengan menggunakan uHPLC-MS/MS

Authors

  • Lusy Indranita Departemen Sains Biomedis, Sekolah Pascasarjana, Universitas YARSI
  • Nunung Ainur Rahmah Departemen Sains Biomedis, Sekolah Pascasarjana, Universitas YARSI
  • Juniarti Juniarti Departemen Sains Biomedis, Sekolah Pascasarjana, Universitas YARSI
  • Moch Abdussalam Lembaga Penelitian, Unviersitas YARSI

https://doi.org/10.33476/ms.v11i1.3940

Keywords:

Anthocyanins, Clitoria ternatea, uHPLC-MS/MS

Abstract

Bunga telang (Clitoria ternatea L) merupakan tanaman yang banyak memiliki efek farmakologis yang cukup baik. Tanaman ini memiliki bunga dengan warna ungu yang menarik yang diduga mengandung senyawa kelompok antosianin. Penelitian ini bertujuan mengidentifikasi senyawa kelompok antosianin dalam ekstrak bunga telang dengan menggunakan ultra high performance liquid chromatography – mass spectroscopy/mass spectroscopy (uHPLC-MS/MS). Pengukuran dengan uHPLC-MS/MS dapat diketahui senyawa kelompok antosianin dalam ekstrak telang adalah Delphinidin-3-(6″-p-coumaroyl)-rutinosida, Sianidin 3-(6″-p-coumaroyl)-rutinosida, Delphinidin-3-(cis-p-coumaroyl-glukosida) dan Cyanidin-3-(p -coumaroyl) glukosa. Oleh karena itu, ekstrak bunga telang telah berhasil dibuktikan mengandung senyawa kelompok antosianin.

Author Biography

Lusy Indranita, Departemen Sains Biomedis, Sekolah Pascasarjana, Universitas YARSI

Biomedical

References

Andriani D dan Murtisiwi L 2018. Penetapan kadar fenolik total ekstrak etanol bunga telang (Clitoria Ternatea L.) dengan spektrofotometri Uv Vis. Cendekia Journal of Pharmacy, 2(1), 32–38. Tersedia pada: https://doi.org/10.31596/cjp.v2i1.15.

Cahyaningsih E, Sandhi PE dan Santoso P 2019. Skrinning fitokimia dan uji aktivitas antioksidan ekstrak etanol bunga telang (Clitoria ternatea L.) dengan metode spektrofotometri UV-VIS. Jurnal Ilmiah Medicamento, 5(1), 51–57. Tersedia pada: https://doi.org/10.36733/medicamento.v5i1.851.

Escher GB, Wen M, Zhang L, Rosso ND, & Granato D 2020. Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L.(butterfly pea) blue petals. Food chemistry, 331, 127341.

Fikayuniar L et al., 2023. Skrinning Fitokimia serta uji karakteristik simplisia dan ekstrak bunga telang (Clitoria ternatea L.) dengan berbagai metode. Jurnal Ilmiah Wahana Pendidikan, 9(15), 308–320. Tersedia pada: https://doi.org/https://doi.org/10.5281/zenodo.8208374.

Gupta GK, Chahal J dan Bhatia M 2015. Clitoria ternatea (L.): Old and new aspects. Journal of Pharmacy Research, 3(11), 2610–2614.

Jelantik RCANP dan Cahyaningsih, E 2022. Potensi antioksidan bunga telang (Clitoria ternatea L.) sebagai penghambat hiperpigmentasi akibat paparan sinar ultraviolet. Scientific Journal of Pharmacy, 18(1), 45–54. Tersedia pada: https://doi.org/https://doi.org/10.20885/jif.vol18.iss1.art5.

Jeyaraj EJ, Lim YY, & Choo WS 2022. Antioxidant, cytotoxic, and antibacterial activities of Clitoria ternatea flower extracts and anthocyanin-rich fraction. Scientific Reports, 12(1), 14890

Jeyaraj JE, Yau YL dan Wee SC 2021. Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. Journal of Food Science and Technology, 58(6), 2054–2067. Tersedia pada: https://doi.org/10.1007/s13197-020-04745-3

Jeyaraj JE, Yau YL dan Wee SC 2022. Antioxidant, cytotoxic, and antibacterial activities of Clitoria ternatea flower extracts and anthocyanin-rich fraction. Scientific Reports, 12(1), 1–12. Tersedia pada: https://doi.org/10.1038/s41598-022-19146-z.

Kazuma K, Noda N, & Suzuki M 2003. Flavonoid composition related to petal color in different lines of Clitoria ternatea. Phytochemistry, 64(6), 1133-1139.

Kazuma K, Noda N, & Suzuki M 2003. Malonylated flavonol glycosides from the petals of Clitoria ternatea. Phytochemistry, 62(2), 229-237

Marpaung AM 2020. Tinjauan manfaat bunga telang (clitoria ternatea l.) bagi kesehatan manusia. Journal of Functional Food and Nutraceutical, 1(2), 63–85. Tersedia pada: https://doi.org/10.33555/jffn.v1i2.30.

Muntaha FM 2019. Analisis bunga telang (Clitoria ternatea) dengan variasi pH metode high performance liquid chromatograph-tandem mass spectrometry (LC-MS/MS) (Doctoral dissertation, Universitas Pasundan).

Nair V, Bang WY, Schreckinger E, Andarwulan N, & Cisneros-Zevallos L 2015. Protective role of ternatin anthocyanins and quercetin glycosides from butterfly pea (Clitoria ternatea Leguminosae) blue flower petals against lipopolysaccharide (LPS)-induced inflammation in macrophage cells. Journal of Agricultural and Food Chemistry, 63(28), 6355-6365.

Sumartini S 2020. Analisis bunga telang (Clitoria ternatea) dengan variasi ph metode liquid chromatograph-tandem mass spectrometry (lc-ms/ms). Pasundan Food Technology Journal (PFTJ), 7(2), 70-77.

Susanti E, & Sari MI 2022. The LC-MS/MS Profile and Total Phenolic Content of Telang Flower Extract and Roselle as NPC1L1 Inhibitor Candidate. Journal of Agromedicine and Medical Sciences, 8(3), 164-169.

Thuy NM, Minh VQ, Ben TC, Thi Nguyen MT, Ha HTN, & Tai NV 2021. Identification of Anthocyanin Compounds in Butterfly Pea Flowers (Clitoria ternatea L.) by Ultra Performance Liquid Chromatography/Ultraviolet Coupled to Mass Spectrometry. Molecules, 26(15), 4539. https://doi.org/10.3390/molecules26154539

Tripathi S, Annapureddy SR, & Sahoo S 2023. Intra-specific pharmacognostic biochemical screening of various populations of Clitoria ternatea L. using liquid chromatography/tandem mass spectrometry product ion scanning (LC-MS2 PIS). Industrial Crops and Products, 203, 117063.

Vidana Gamage GC, Lim YY, & Choo WS 2021. Anthocyanins from Clitoria ternatea flower: Biosynthesis, extraction, stability, antioxidant activity, and applications. Frontiers in Plant Science, 12, 792303.

Widowati W, Darsono LLucianus J, Setiabudi E, Obeng SS, Stefani S, & Rizal R 2023. Butterfly pea flower (Clitoria ternatea L.) extract displayed antidiabetic effect through antioxidant, anti-inflammatory, lower hepatic GSK-3β, and pancreatic glycogen on Diabetes Mellitus and dyslipidemia rat. Journal of King Saud University-Science, 35(4), 102579.

Zahara M 2022. Deskripsi kembang telang dan manfaatnya. Jurnal Jeumpa, 9(2), 719–728. Tersedia pada: https://doi.org/https://doi.org/10.33059/jj.v9i

Zona AHA 2021. Analisis anti inflamasi senyawa aktif bunga Clitoria ternatea L dengan pendekatan quantitative structure-activity relationship dan in silico. Pesquisa Veterinaria Brasileira. Akademi Analis Farmasi dan Makanan Putra Indonesia Malang. Tersedia pada: http://www.ufrgs.br/actavet/31-1/artigo552.pdf

Downloads

Published

2024-06-19

How to Cite

Indranita, L., Ainur Rahmah, N., Juniarti, J., & Abdussalam, M. (2024). Identifikasi Senyawa Kelompok Antosianin dalam Ekstrak Bunga Telang (Clitoria ternatea) dengan menggunakan uHPLC-MS/MS. Majalah Sainstekes, 11(1), 036–044. https://doi.org/10.33476/ms.v11i1.3940

Issue

Section

Articles

Most read articles by the same author(s)