Penentuan Jenis Dengan Analisis Gen 16SrRNA dan Uji Daya Reduksi Bakteri Resisten Merkuri Yang Diisolasi Dari Feses Pasien Dengan Tambalan Amalgam Merkuri di Puskesmas Bahu Manado
Authors
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Fatimawali Umar
Faculty of Medicine
University of Sam Ratulangi, Manado
Keywords:
Bakteri resisten merkuri, Gen 16SrRNA, reduksi merkuri, CV-AASAbstract
Paparan merkuri secara kontinyu dalam saluran pencernaan, dapat menyebabkan kresistensi bakteri terhadap merkuri. Bakteri resisten merkuri bermanfaat pada proses detoksifikasi merkuri anorganik dengan mereduksinya menjadi logam merkuri yang tidak toksik. Penelitian ini bertujuan untuk menganalisis gen 16SrRNA dan menguji daya reduksinya terhadap merkuri HgCl2 dari bakteri resisten merkuri anorganik Isolat F2.1 dan F2.2 yang diisolasi dari feses pasien dengan tambalan amalgam gigi di Puskesmas Bahu Manado. Analisis Gen 16SrRNA menggunakan metode Polymerase chain reaction (PCR) dan kadar merkuri dianalisis dengan menggunakan metode Cold-Vapor Atomic Absorption Spectrophotometry (CV-AAS). Hasil BLAST urutan nukleotida gen 16SrRNA menunjukkan bahwa kedua isolat bakteri tersebut mempunyai kemiripan 100% terhadap gen 16SrRNA bakteri Escherichia coli yang terdapat pada GenBank. Hasil analisis daya reduksi merkuri diperoleh bahwa dalam waktu 1, 12, dan 24 jam dapat menurunkan kadar merkuri dalam media berturut-turut untuk isolat F2.1: 82,2%, 87,1% dan 99,2% dan untuk isolat F2.2: 79,5%, 89,2% dan 99,3%.Hasil penelitian menunjukkan bahwa bakteri isolat F2.1 dan F2.2 yang diisolasi dari feses ialah bakteri Escherichia coli dan dapat mereduksikan HgCl2 hampir 100% dalam waktu 24 jam sehingga bakteri tersebut dapat digunakan pada penelitian selanjutnya untuk proses detoksifikasi merkuri organik.
Continuous exposure to mercury in the digestive tract, can cause mercury-resistance bacteria. Mercury resistance bacteria are useful in detoxifying processes of inorganic mercury to the reduct form of non toxic metallic mercury.This study aims to analyze 16SrRNA gene and test for mercury reduction ability of inorganic mercury resistant bacteria isolates F2.1 and F2.2, isolated from feces of patients with tooth amalgam at Puskesmas Bahu in Manado. 16SrRNA gene analysis was done using polymerase chain reaction (PCR) and mercury levels were analyzed by using the method of Cold - Vapor Atomic Absorption Spectrophotometry (CV - AAS). BLAST results of nucleotide sequence of 16SrRNA gene showed that both the bacterial isolates had 100% similarity to the 16SrRNA gene of Escherichia coli bacteria found in GenBank. The results of the analysis showed that the reduction ability of mercury in 1 , 12 , and 24 hours can reduce levels of mercury in a row for a media F2.1 isolates: 82.2%, 87.1% and 99.2% and for isolates F2.2: 79.5%, 89.2% and 99.3%. The results showed that the bacterial isolates F2.1 and F2.2 isolated from fecal is Escherichia coli bacteria and may reduce the HgCl2 almost 100% within 24 hours so that the bacteria can be used in future studies to inorganic mercury detoxification process.
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