Antioxidant Activity of Methanol Extract from Several Indonesian Green Teas


  • adelina simamora Department of Biochemistry Faculty of medicine Krida Wacana Christian University
  • Michael Steven Faculty of medicine Krida Wacana Christian University
  • Adit Widodo Santoso Faculty of medicine Krida Wacana Christian University
  • Flora Rumiati Deparment of Physiology Faculty of medicine Krida Wacana Christian University
  • Kris Herawan Timotius Department of Biochemistry Faculty of medicine Krida Wacana Christian University


Antioxidant, DPPH, green tea, Indonesia, reducing power


As a common beverage, green tea is supposed to have beneficial health effect, such as antioxidant. At present, there are many green teas available in the market in Jakarta. Their quality, in their antioxidant activity, should be proved. This study aimed to investigate the antioxidant activity of extracts of green tea products commercially available in Jakarta. Four green tea samples from different factories (coded as A, B, C, and D) were selected and macerated using methanol. DPPH radical scavenging activity, reducing power ability and total antioxidant capacity were used to measure the antioxidant activity. The total phenolic content (TPC) was also determined. The studied green teas had varied TPC from 23.80 to 84.03 mG GAE/g extract, ranked as B more than D more than A more than C. All samples exhibited various but strong antioxidant activity by DPPH assay, even better than standards ascorbic acid and butylated hydroxytoluene (BHT). However, all extracts showed similar activities in their reducing power ability and total antioxidant capacity, with activities less than standards. These findings confirm that the quality of the Indonesian commercial green teas were heterogeneous both in TPC or DPPH scavenging capacity. But, there is an indication that they are good as an antioxidant containing beverage.

Author Biography

adelina simamora, Department of Biochemistry Faculty of medicine Krida Wacana Christian University

Senior Lecturer

Department of Biochemistry

Faculty of medicine

Krida Wacana Christian University


ADJIMANI, J. P. & ASARE, P. 2015. Antioxidant and free radical scavenging activity of iron chelators. Toxicology Reports, 2, 721-728.

ALAM, M. N., BRISTI, N. J. & RAFIQUZZAMAN, M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21, 143-152.

ARAB, L., KHAN, F. & LAM, H. 2013. Tea consumption and cardiovascular disease risk. The American Journal of Clinical Nutrition, 98, 1651S-1659S.

DHULL, S. B., KAUR, P. & PUREWAL, S. S. 2016. Phytochemical analysis, phenolic compounds, condensed tannin content and antioxidant potential in Marwa (Origanum majorana) seed extracts. Resource-Efficient Technologies, 2, 168-174.

Duh PD 1998. Antioxidant Activity Of Burdock (Arctium Lappa Linne): Its Scavenging Effect On Free-Radical And Active Oxygen. ?Journal Of The American Oil Chemists' Society, 75, 455-461

GENWALI, G. R., ACHARYA, P. P. & RAJBHANDARI, M. 2013. Isolation of Gallic Acid and Estimation of Total Phenolic Content in Some Medicinal Plants and Their Antioxidant Activity. Nepal Journal of Science and Technology, 14, 95-102.

GULCIN, I., ELMASTAS, M. & ABOUL-ENEIN, H. Y. 2007. Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytotherapy Research, 21, 354-361.

JAYAPRAKASHA, G., SINGH, R. & SAKARIAH, K. 2001. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chemistry, 73, 285-290.

KAUR, L., JAYASEKERA, S. & MOUGHAN, P. J. 2014. Antioxidant quality of tea (Camellia sinensis) as affected by environmental factors. Processing and Impact on Antioxidants in Beverages, 121-9.

KHATOON, M., ISLAM, E., ISLAM, R., RAHMAN, A. A., ALAM, A. K., KHONDKAR, P., RASHID, M. & PARVIN, S. 2013. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Research Notes, 6, 1-7.

KHENNOUF, S., AMIRA, S., ARRAR, L. & BAGHIANI, A. 2010. Effect of some phenolic compounds and quercus tannins on lipid peroxidation. World Applied Sci J, 8.

KOLEVA, I. I., VAN BEEK, T. A., LINSSEN, J. P., GROOT, A. D. & EVSTATIEVA, L. N. 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis, 13, 8-17.

KRISTANTI, R. & PUNBUSAYAKUL, N. Antioxidant and antimicrobial activity of commercial green tea in Chiang Rai. Asia Pacific Symposium on Assuring Quality and Safety of Agri-Foods 837, 2008. 53-58.

MAKRIS, D. P., BOSKOU, G. & ANDRIKOPOULOS, N. K. 2007. Polyphenolic content and in vitro antioxidant characteristics of wine industry and other agri-food solid waste extracts. Journal of Food Composition and Analysis, 20, 125-132.

OYAIZU, M. 1986. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition, 44.

Paixão N, Perestrelo R, Marques JC, Câmara JS 2007. Relationship Between Antioxidant Capacity And Total Phenolic Content Of Red, Rosé And White Wines. Food Chemistry, 105, 204-214.

Patlevi? P, Vašková J, Švorc P, Vaško L, Švorc P 2016. Reactive Oxygen Species And Antioxidant Defense In Human Gastrointestinal Diseases. Integrative Medicine Research, 5, 250-258.

PISOSCHI, A. M., POP, A., CIMPEANU, C. & PREDOI, G. 2016. Antioxidant Capacity Determination in Plants and Plant-Derived Products: A Review. Oxidative Medicine and Cellular Longevity, 2016.

SHEN, Q., ZHANG, B., XU, R., WANG, Y., DING, X. & LI, P. 2010. Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01. Anaerobe, 16, 380-386.

SPADIENE, A., SAVICKIENE, N., IVANAUSKAS, L., JAKSTAS, V., SKESTERS, A., SILOVA, A. & RODOVICIUS, H. 2014. Antioxidant effects of Camellia sinensis L. extract in patients with type 2 diabetes. Journal of Food and Drug Analysis, 22, 505-511.

VUONG, Q. V. 2014. Epidemiological evidence linking tea consumption to human health: a review. Critical Reviews in Food Science and Nutrition, 54, 523-536.

WANG, D., WANG, L.-J., ZHU, F.-X., ZHU, J.-Y., CHEN, X. D., ZOU, L., SAITO, M. & LI, L.-T. 2008. In vitro and in vivo studies on the antioxidant activities of the aqueous extracts of Douchi (a traditional Chinese salt-fermented soybean food). Food Chemistry, 107, 1421-1428.

WANG, X., LIU, Q., ZHU, H., WANG, H., KANG, J., SHEN, Z. & CHEN, R. 2017. Flavanols from the Camellia sinensis var. assamica and their hypoglycemic and hypolipidemic activities. Acta Pharmaceutica Sinica B, 7, 342-346.

YUAN, J.-M. 2013. Cancer prevention by green tea: evidence from epidemiologic studies. The American journal of clinical nutrition, 98, 1676S-1681S.






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