Suplementasi bFGF (basic Fibroblast Growth Factor) meningkatkan kecepatan migrasi sel kultur HDF (Human Dermal Fibroblast) pada model luka in vitro

Yurika Sandra

Abstract


Fibroblasts are the key cell responsible for wound healing. To accelerate wound healing, molecules are needed to improve the proliferation and migration of fibroblasts. bFGF is a growth factor commonly used as a supplement in cell culture to improve proliferation and maintain stemness cell.  This study was to verify the effect of bFGF on HDF migration.

This study was an experimental design using four groups of fibroblasts sample. The intervention groups were cultured with bFGF and bFGF+DMSO combination, where as the control groups where cultured with standard supplement and DMSO. Fibroblasts were obtained from Stem Cell Laboratory at Yarsi University. A pipette tip was used to prepare a scratch across the layer of fibroblast. The average extent of wound closure was evaluated by measuring the width of the wound. The speed of migrating cells into the wound area was examined and photographed. Cell migration was assessed by microphotography. Data analysis was performed with paired student t-test.

bFGF enhanced migration of fibroblast after 24 hours about  40%, where as DMSO 2% inhibited migration of fibroblast about 40,83%. Fibroblast was supplemented DMSO2% + bFGF 8ng/ml combination showed similar with untreated group.

The result of this study indicate that the bFGF may be useful in management of  wounds

 


Keywords


Fibroblast kulit manusia, bFGF, Migrasi, DMSO

Full Text:

PDF

References


Applegate, L.A., Scaletta, C., Hirt-Burri, N., Raffoul, W., Pioletti, D., 2009. Whole-cell bioprocessing of human fetal cells for tissue engineering of skin. Skin Pharmacol. Physiol. 22, 63–73. doi:10.1159/000178865

Bainbridge, P., 2013. Wound healing and the role of fibroblasts. J. Wound Care 22, 407–408, 410–412. doi:10.12968/jowc.2013.22.8.407

Ganguly, A., Yang, H., Sharma, R., Patel, K.D., Cabral, F., 2012. The Role of Microtubules and Their Dynamics in Cell Migration. J. Biol. Chem. 287, 43359–43369. doi:10.1074/jbc.M112.423905

Krishnamoorthy, J.R., Sumitira, S., Ranjith, M.S., Gokulshankar, S., Ranganathan, S., Mohanty, B.K., Prabhakaran, G., 2012. An in vitro study of wound healing effect of a poly-herbal formulation as evidenced by enhanced cell proliferation and cell migration. Egypt. Dermatol. Online J. 8, 1.

Lampugnani, M.G., Pedenovi, M., Niewiarowski, A., Casali, B., Donati, M.B., Corbascio, G.C., Marchisio, P.C., 1987. Effects of dimethyl sulfoxide (DMSO) on microfilament organization, cellular adhesion, and growth of cultured mouse B16 melanoma cells. Exp. Cell Res. 172, 385–396.

Moulin, V., Castilloux, G., Jean, A., Garrel, D.R., Auger, F.A., Germain, L., 1996. In vitro models to study wound healing fibroblasts. Burns J. Int. Soc. Burn Inj. 22, 359–362.

Schmidt, A., Ladage, D., Schinköthe, T., Klausmann, U., Ulrichs, C., Klinz, F.-J., Brixius, K., Arnhold, S., Desai, B., Mehlhorn, U., Schwinger, R.H.G., Staib, P., Addicks, K., Bloch, W., 2006. Basic Fibroblast Growth Factor Controls Migration in Human Mesenchymal Stem Cells. Stem Cells 24, 1750–1758. doi:10.1634/stemcells.2005-0191

Wang, C.-C., Lin, S.-Y., Lai, Y.-H., Liu, Y.-J., Hsu, Y.-L., Chen, J.J.W., 2012. Dimethyl sulfoxide promotes the multiple functions of the tumor suppressor HLJ1 through activator protein-1 activation in NSCLC cells. PloS One 7, e33772. doi:10.1371/journal.pone.0033772

Wu, J., Huang, G.T.-J., He, W., Wang, P., Tong, Z., Jia, Q., Dong, L., Niu, Z., Ni, L., 2012. Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla. J. Endod. 38, 614–622. doi:10.1016/j.joen.2012.01.014




DOI: https://doi.org/10.33476/jky.v25i2.337

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 YARSI Medical Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

__________________________________________________________________________________________

Copyright of Jurnal Kedokteran YARSI.

Powered by OJS.

Creative Commons License

This work is licensed under a Creative Commons Attribution- NonCommercial 4.0 International License.