Pemanfaatan darah manusia yang kadaluarsa sebagai pengganti darah domba dalam pembuatan media Agar Darah Plat (ADP)
Authors
-
Titiek Djannatun
Department of Microbiology YARSI UNIVERSITY School of Medicine, Jakarta
-
Jekti T Rochani
Department of Microbiology YARSI UNIVERSITY School of Medicine, Jakarta
-
Riyani Wikaningrum
Department of Microbiology YARSI UNIVERSITY School of Medicine, Jakarta
-
Dian Widiyanti
Department of Microbiology YARSI UNIVERSITY School of Medicine, Jakarta
-
Abdul Rahim Pane
Department of Microbiology YARSI UNIVERSITY School of Medicine, Jakarta
Keywords:
blood agar media, nearly expired human blood, expired human bloodAbstract
The use of blood agar media to grow and to isolate the pathogenic bacteria, and to compare the hemolytic ability of the bacteria is well known. The aim of this study was to investigate the possibility of using expired human blood as substitute for the sheep blood agar media. In this study five days before expired and five days after expired human blood were washed once, twice and centrifuged. Sheep blood was used as positive control and five days after and before expired blood without treatment was used as negative control. The prepared media were used to grow six isolates wild strain bacteria (Staphylococcus aureus, Streptococcus B haemolyticus,Streptococcus y haemolyticus, Streptococcus pneumoniae, Vibrio El Tor and Clostridium perfringens). The result indicated that blood agar medium, prepared using human blood which was nearly expired or expired, have the same results in culture growth of the tested bacteria compared to the standard blood agar had media using sheep blood.References
Anonymous 1982. The Oxoid Manual of Culture Media, Ingredients and other Laboratory Services. 5th ed. UK. Oxoid Limited
Bisming W and Amstberg G 1993. Colour Atlas for the Diagnosis of Bacterial Pathogen in animals. Paul Parey Scientific Publisher. Hamburg.
Brooks GF, S. Butel and SA Morse 2002. Jawetz, Melnick & Adelberg’s Mikrobiologi Kedokteran (Medical Microbiology). Ed. 1. Penerjemah dan Editor Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Penerbit Salemba Medika.
Carter GR 1986. Essentials of Veterinary Bacteriology and Mycology.3rd Ed. Lea and Febiger, Philadelphia, USA.
Chadwick PR and B.A. Oppenheim 1995. Neomycin Blood Agar As A selective Medium for Vancomycin Resistant Enterococcus faecium. J. of Clin. Pathol. 48(11): 1068-1070.
Cheesbrough M 1991. Medical Laboratory Manual for Tropical Countries, volume II: Microbiology. Cambridge. ELBS.
Cottral GE 1978. Manual of Standardized Methods for Veterinary Microbiology. Cornell University Press. London.
Fallon D, K.J. Nye, B. Gee, S. Messer, R.E. Waren, and N. Andrew. 2000. A Comparison of Columbia Blood Agar With Or Without Oxolinic Acid/Metronidazole for The Isolation of B-haemolytic Streptococcus from throat Swabs. J. med. Microbiol. (49): 941-942.
Hof H and R. Dörries 2002. Medizinische Mikrobiologie. 2., Korrigierte Auflage. Georg Thieme Verlag Stuttgart.
Jarp J 1990. Staphylococcus from Mastitis in Ruminants: Virulence Properties and Classification of species. Dissertation Abstracts International, C, Worlwide. (51): 2, 209 C.
Mims CA 1982. The Pathogenesis of Infectious disease. 2nd Ed. Acad. Press. London. New York. San Francisco. Sao Paulo. Sydney. Tokyo. Toronto. Pp.56-81.
Norrel SA & K.E. Messley 1997. Microbiology Laboratory Manual: Principles and Applications. New Jersey. Prentice Hall.
Wibawan IWT and Laemmler 1990. Propeties of Group B streptococci With Protein Surface antigens X and R. J. Clin. Microbiol. (28): 2834-2836.
Wistreich GA 1997. Microbiology Laboratory: Fundamentals and Applications. New Jersey. Prentice Hall.
