Efek Pentagamavunon-0 terhadap Konsentrasi cAMP dan Progesteron pada Kultur Sel Luteal yang mengandung Teofilin
Authors
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Endang Purwaningsih
Department of Anatomy (Biology), Faculty of Madicine, YARSI University, Jakarta
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Sri Kadarsih Soejono
Department of Physiology, Faculty of Medicine, Gadjah Mada University, Yogyakarta
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Djaswadi Dasuki
Department of Obstetric and Gynecology, Faculty of Madicine, Gadjah Mada University,
Yogyakarta
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Edy Meiyanto
Department of Molecular Biology, Faculty of Pharmacy, Gadjah Mada University,
Yogyakarta
Keywords:
signal transduction, curcumin analog, culture plate, ELISA, RIAAbstract
Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2? with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2? on cAMP and progesterone concentrations showed no significant difference compared to the control. Theophylline increased the cAMP and progesterone concentration significantly but not to LH stimulation. PGV-0 did not inhibit cAMP concentration but PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.References
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