Efek kurkumin dan pentagamavunon-0 terhadap viabilitas kultur sel luteal


  • Endang Purwaningsih Department of Anatomy (Biology), Faculty of Medicine, YARSI University, Jakarta



curcumin, curcumin analog, proliferation, luteal cells


The purpose of this study was to investigate the effect of curcumin and pentagamavunonn-0 (PGV-0) on the viability of cultured luteal cells (percent of viable cells). The cultured luteal cells were obtained from corpus luteum of induced 12 immature Sprague Dawley rats by a single 10 IU Pregnat Mare’s Serum Gonadotropin. Subject in this study was 4 days old corpus luteum which was cultured in Minimal Essential Medium (MEM) containing 10% Fetal Bovine Serum. This experiment consisted of 12 groups, e.g. one group as control (solvent), three groups as control with LH and or PGF2 stimulation, and eight groups treated with 100 ?M curcumin and PGV-0 in three replicates. Evaluation was conducted after 24 hours of treatment by observation of viable cells with MTT method. The result showed that the viability of cultured luteal cells with curcumin or PGV-0 only was not significantly different compared to control goups. However, curcumin and PGV-0 on cultured luteal cells stimulated by LH and or PGF2 showed significant differences compared to control or LH and PGF2 only.


Aggarwal BB, Kumar A, and Bharti AC 2003. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res 23(IA): 363-398.

Anto RJ 2002. Curcumin (diferuloylmethane) induced apoptosis through activation of caspase-8, BID cleavage and cytochrome c release: Its suppression by actopic expression of Bcl-2 and Bcl-xL, Carcinogenesis 23(1): 143-150.

Chan TA, Morin PJ, Vogelstein B, and Kinzler KW 1998. Mechanism Underlying Nonsteroidal Antiinflamatory Drug-Mediated Apoptosis, Proc.Natl. Acad.Sci. 95: 681-686.

Da’i M 2003. Uji Aktivitas Antiproliferatif Pentagamavunon-0 Terhadap sel Ragi, sel Hela dan sel Myeloma, Tesis, Program Pascasarjana,

Universitas Gadjah Mada, Yogyakarta.

Da’i M, Meiyanto E, Supardjan AM, Jenie UA, Kawaichi M 2007. Potensi Antiproliferatif Analog Kurkumin Pentagamavunon terhadap Sel Kanker Payudara T47D. Artocarpus 7 (1): 14-20.

Doyle A, and Griffiths JB 2000. Cell and Tissue Culture For Medical Research, John Wiley and Sons Ltd,New York.

Freshney RI 1990. Culture of Animal Cells. A manual of Basic Technique, Second Ed, Alan R Lies Inc, New York

Garg SK 1974. Effect of Curcuma longa (Rhizome) on Fertility in Experimental Animal, Planta Medica 26: 225-227.

Huang MT, Ma W, lou JR, Lu YP, Chang R, Newmark H, Conney AH 1995. Inhibitory Effects of curcumin on Tumorogenesis in Mice. In: Pramono S, Jenie UA, Sudibyo RS, Gunawan D Ed. Recent Developments in Curcumin Pharmacochemistry, Proceeding Of The International Symposium on Curcumin Pharmacochemis try (ISCP), Aditya Media.,Yogyakarta. 47 – 61.

Kakar SS and Roy D 1994. Curcumin inhibits TPA induced expression of c-fos, c-jun and c-myc proto-oncogens messenger RNAs in mouse skin,

Cancer Lett 87(1): 85-89.

Khan MI, Rosberg S, Lahav M, Lamprecht SA, Selstam G, Herlitz H, Ahren K 1979. Study of the mechanism of action of the inhibitory effect of PGF2 on cyclic AMP accumulation in rat korpora lutea of various ages.Biol Reprod 21:1175.

Khar A, Ali AM, Pandasaradhi, Begum Z, and Anjum R 1999. Antitumor activity of curcumin is mediated through the induction of apoptosis in

AK-5 tumor cells. FEBS Letter: 445: 165-168.

King RJB 2000. Cancer Biology, 2ed., Pearson Education Limited, London.

Majeed M, Badmaev V, Shivakumar U, Rajendran R 1995. Curcuminoids: Antioxidant Phytonutrients, Piscataway Inc, New York; 1995.

Meiyanto E 1999. Kurkumin sebagai obat kanker. Menelusuri Mekanisme Aksinya Majalah Farmasi Indonesia 10 (94) : 224-236.

Mossman T 1983. Rapid Colorimetric assay for Celluler growth and Survival Application to Proliferation and Cytotoxicity assays. J Immune

Methode 65: 65-95.

Mukhopadhyay A, Banerjee S 2001. Stafford of cell proliferation correlates with down-regulation of cyclin DI expression and CDK4-mediated retinoblastoma protein phosphorylation, Oncogene 21(57): 8852-8861

Niswender GD, Juengel JL, Silva PJ, Rollyson MK, Mc Intush EW 2000. Mechanism Controlling the Function and Life Span of the Corpus Luteum. Physiol Rev 80 (1) : 1 – 29.

Niswender GD 2002. Molecular Control of Luteal secretion of Progesterone. Review. Reproduction 123: 333-339.

Sardjiman 2000. Synthesis of Some New Series of Curcumin Analogues, Antioxidative, antiimflamatory, antibacterial Activities, and Qualitative - Structure Relationship. Dissertation, Gadjah Mada University, Yogyakarta.

Sigma 1999. Biochemicals and Reagents for Life Science Research. Sigma Aldrich C0, Singapore.

Susanto A 2004. Daya Antiproliferasi Pentagamavunon-0 Terhadap sel Kanker payudara T47D, Skripsi, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.

Tim Molnas 2001. Uji Ketoksikan Akut, Keteratogenikan dan Ulserogenik Senyawa PGV0, PGV-1, dan HGV-1 pada Tikus dan Kelinci, 128, Laporan Penelitian Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Timmerman H 1995. New perspective for anti - inflammatory drugs. In: Pramono S, Jenie UA, Sudibyo RS, Gunawan D Ed. Recent Development in Curcumin Pharmacochemistry, Proceeding International Symposium on Curcumin Pharmacochemistry (ISCP). Aditya Media,

Yogyakarta. 1-12.

Tonnesen HH and Karlsen J 1995. High Performance Liquid Chromatography of Curcumin and Related Compound, J Chrom 259: 367 – 371.

Weinberg RA 1995. The Retinoblastoma Protein and Cell Cycle Control. Cell 71: 505-514.






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