Biosintesis Antigen Permukaan Hepatitis B “HBsAg100” pada Escherichia coli dalam Rangka Produksi Protein Rekombinan sebagai Model Imunogen untuk Menghasilkan Antibodi
Authors
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Slamet Riyadi
Student of Veterinary Science Study Program, School of Postgraduate Studies, Bogor Agricultural
University
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Rarah RA Maheswari
Faculty of Animal Husbandry, Bogor Agricultural University
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Mirnawati Sudarwanto
Faculty of Veterinary Science, Bogor Agricultural University
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Fransiska RZ
Fakulty of Technology Agricultural, Bogor Agricultural University
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Muhamad Ali
Chairman Laboratory of Microbiology & Biotechnology Faculty of Animal Husbandry, University of Mataram, Lombok
Keywords:
Ekspresi protein, protein rekombinan, purifikasi, HBsAg100Abstract
Biosintesis protein rekombinan melalui Escherichia coli memberikan alternatif untuk menghasilkan protein antigen yang bermanfaan bagi kepentingan kesehatan yang bebas dari protein manusia. Penelitian ini menggabungkan fragmen DNA dari antigen permukaan virus Hepatitis B dengan gen penyandi enzim gluthation-S-transferase (GST) di dalam plasmid p GEX-4T-2 yang di ekspresikan di dalam sel-sel Escherichia coli. Polypeptida dengan berat molekul sekitar 34,8 kDa telah diproduksi dan diidentifikasi sebagai protein gabungan GST-HB100. Protein gabungan tersebut kemudian dimurnikan menggunakan kolum GSTrap yang disambung dengan kolum HiTrap. Selanjutnya, protein hasil pemurnian tersebut diharapkan bisa digunakan sebagai bahan vaksin atau untuk menghasilkan antibodi.References
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