The Role of ZFH-04269, Pilicides ec240, and Mannosides as FimH Adhesins Inhibitor of Uropathogenic Escherichia coli (UPEC) and Multiplex Real-Time PCR as a Potential Method in Diagnosing Urinary Tract Infections
Authors
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Ignatius Ivan
Atma Jaya Catholic University of Indonesia
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Harvey Sudharta
Atma Jaya Catholic University of Indonesia
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Nicolas Daniel Widjanarko
Atma Jaya Catholic University of Indonesia
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Maureen Miracle Stella
Atma Jaya Catholic University of Indonesia
http://orcid.org/0000-0003-2932-3498
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Kevin Tandarto
Atma Jaya Catholic University of Indonesia
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Mariani Santosa
Atma Jaya Catholic University of Indonesia
Keywords:
Escherichia coli, UTI, Anti-adhesive, Multiplex Real-time PCRAbstract
Urinary tract infections (UTIs) can be difficult to diagnose and therefore a quicker and cheaper method but still having a high reliability result is needed. Multiplex real-time PCR can diagnose UTI better, as it has a high accuracy and time-efficiency. In other hand, as many infection is caused by bacterial attachment to cells, anti-adhesion therapeutics can be an option for treatment methods. Our objective is to analyse whether real-time multiplex PCR and anti-adhesive therapeutics can become the best methods for diagnosis and treatment options respectively. Results showed that it is feasible to identify uropathogens with more than 90% accuracy by using multiplex real-time PCR; while modified isoquinolone-mannoside is proved to have the highest FimH affinity and potency of any antagonists reported to date. Finally, we conclude that multiplex real-time PCR and the use of ZFH-04269, pilicides ec240, and mannosides as FimH adhesins inhibitor might be applicable as novel diagnostic method and treatment for UTIs.
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