Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt

Adit Widodo Santoso, Adelina Simamora, Adelina Simamora, Kris Herawan Timotius, Kris Herawan Timotius


Myristica fragrans Houtt (nutmeg) is used as a spice and flavour for food and beverages. It has been traditionally used to treat a number of medical conditions, including diabetes mellitus. The study was undertaken to scientifically validate the traditional use of mace from M. Fragrans. The objectives of this study were to evaluate α-glucosidase inhibition, antioxidant and antibacterial activities of water extract (WE) and essential oil (EO) from M. fragrans mace.  Both WE and EO were evaluated for their α-glucosidase inhibitory activities in vitro and their antioxidant activities based on DPPH radical scavenging assay. Standard compounds were used for every test. Total phenolic and flavonoid contents of both extracts were also determined. The extracts were also tested for their antibacterial activities against six different bacteria by a well diffusion method. Both extracts showed inhibition activities against α-glucosidase, with WE showed stronger activity than EO (IC50 = 1.86 and 8.15 mg/ml). Good radical scavenging activities were observed for both extracts, with WE showed stronger activity than EO (IC50 = 1.51 and 4.59 mg/ml). WE showed higher content in phenolic than EO (47.84 and 37.21 mg GAE/100 g DW). Flavonoid content in WE was also higher than EO (215.36 and 30.12 mg RE/ml). Based on the well diffusion method, only EO exhibited antibacterial activities, with inhibition zone in the range 1.03 – 1.30 mm.  The strongest activity was observed against Staphylococcus mutans. The results indicate WE and EO can be exploited further for pharmacological uses, in particular for their antidiabetic and antioxidant activities. 


Myristica fragrans, α-glucosidase inhibition, antioxidant, antibacteria

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Assa, J. R., Widjanarko, S. B., Kusnadi, J., & Berhimpon, S. (2014). Antioxidant potential of flesh, seed and mace of nutmeg (Myristica fragrans Houtt). International Journal of ChemTech Research, 6(4), 2460-2468.

Brand-Williams, W., Cuvelier, M.-E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol, 28(1), 25-30.

Champasuri, S., & Itharat, A. (2016). Bioactivities of ethanolic extracts of three parts (Wood, nutmeg and mace) from Myristica fragrans Houtt. J Med Assoc Thai, 99, S124-S130.

De Luca, V., Salim, V., Atsumi, S. M., & Yu, F. (2012). Mining the biodiversity of plants: a revolution in the making. Science, 336(6089), 1658-1661.

Dong, H.-Q., Li, M., Zhu, F., Liu, F.-L., & Huang, J.-B. (2012). Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α-amylase linked to type 2 diabetes. Food Chem, 130(2), 261-266.

Gao, J., Xu, P., Wang, Y., Wang, Y., & Hochstetter, D. (2013). Combined effects of green tea extracts, green tea polyphenols or epigallocatechin gallate with acarbose on inhibition against α-amylase and α-glucosidase in vitro. Molecules, 18(9), 11614-11623.

Gupta, A. D., Bansal, V. K., Babu, V., & Maithil, N. (2013). Chemistry, antioxidant and antimicrobial potential of nutmeg (Myristica fragrans Houtt). J Genet Eng Biotechnol, 11(1), 25-31.

Mai, T. T., Thu, N. N., Tien, P. G., & Chuyen, N. V. (2007). Alpha-Glucosidase Inhibitory and Antioxidant Activities of Vietnamese Edible Plants and Their Relationships with Polyphenol Contents. J Nutr Sci Vitaminol, 53(3), 267-276.

Malapermal, V., Botha, I., Krishna, S. B. N., & Mbatha, J. N. (2015). Enhancing antidiabetic and antimicrobial performance of Ocimum basilicum, and Ocimum sanctum (L.) using silver nanoparticles. Saudi J Biol Sci.

Oboh, G., Agunloye, O. M., Adefegha, S. A., Akinyemi, A. J., & Ademiluyi, A. O. (2015). Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study. J Basic Clin Physiol Pharmacol, 26(2), 165-170.

Oboh, G., Ogunsuyi, O. B., Ogunbadejo, M. D., & Adefegha, S. A. (2016). Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose. J Food Drug Anal, 24(3), 627-634.

Paixão, N., Perestrelo, R., Marques, J. C., & Câmara, J. S. (2007). Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines. Food Chem, 105(1), 204-214.

Shafiei, Z., Shuhairi, N. N., Md Fazly Shah Yap, N., Harry Sibungkil, C. A., & Latip, J. (2012). Antibacterial activity of Myristica fragrans against oral pathogens. Evid Based Complement Alternat Med, 2012.

Shukla, S., Park, J., Kim, D.-H., Hong, S.-Y., Lee, J. S., & Kim, M. (2016). Total phenolic content, antioxidant, tyrosinase and α-glucosidase inhibitory activities of water soluble extracts of noble starter culture Doenjang, a Korean fermented soybean sauce variety. Food Control, 59, 854-861.

Singleton, V., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic, 16(3), 144-158.

Sulaiman, S. F., & Ooi, K. L. (2012). Antioxidant and anti food-borne bacterial activities of extracts from leaf and different fruit parts of Myristica fragrans Houtt. Food Control, 25(2), 533-536. doi:

Velioglu, Y., Mazza, G., Gao, L., & Oomah, B. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of agricultural and food chemistry, 46(10), 4113-4117.

WHO. (2015). World health statistics 2015. Geneva, Switzerland: World Health Organization.



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