Deteksi Mutasi Gen Gyrase A Porphyromonas Gingivalis Resisten terhadap Ciprofloxacin berdasarkan teknik Polymerase Chain Reaction

Irene E.Rieuwpassa, Mochammad Hatta

Abstract


One of resistance mechanisms to ciprofloxacin shown by bacterium Porphyromonas gingivalis isolated from periodontitis patients is mutations of genes through changes in DNA topoisomerase. Ciprofloxacin is an effective antimicrobial for Gram-negative bacteria effectively used for clinical infections treatment. The purpose of this research was to determine the gene mutation of P. gingivalis on periodontitis patients in relation to the resistance to ciprofloxacin by using disc diffusion and, followed by RFLP-PCR on P.gingivalis samples. Based on sensitivity tests by diffusion method, it was shown that the sensitive samples (n =13) had the highest inhibition zone diameter (mean= 25 mm), while the intermediate samples (n=1) was 19 mm. Examination using RFLP-PCR for 14 samples did not reveal any mutation of gyrase A gene.

Keywords


gyrase A; Porphyromonas gingivalis; ciprofloxacin

Full Text:

PDF

References


Ansubel F, Donald MC et al., 1995. Short Protocols in Moleculer Biology: The Polymerase Chain Reaction, third edition, John Wiley and Sons, Inc, USA.

Bachrach G, Altman H, Kolenbrander PE et al., 2007. Resistance of Porphyromonas gingivalis ATCC 33277 to Direct Killing by Antimicrobial Peptides is Protease Independent. Institute of Dental Sciences, The Hebrew UniversityHadassah School of Dental Medicine.Jerusalem,Israel.

BioLabs, Restriction Endonucleases, New England Biolabs. Diakses 28 Januari 2007.

Brooks GF, Butel SJ dan Morse SA 2001. Mikrobiologi Kedokteran. Penerjemah dan Editor. Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Salemba Medika. Jakarta.

Buhlin Kare, Gustafsson Anders, Pockley AG, Frostegard Johan Klinge Bjorn 2003. Risk Faxtors for Cardiovascular Disease in Patients with

Periodontitis, European Heart J.24: 2099-2107

Burt BA 1996. Epidemiology of Periodontal Disease. J.Periodontal.67:935-945.

Burton GRW, Engelkirk 2004. Microbiology for the Health Sciences. Sevent Edition. Lippincott William & Wilkims, USA.

Collins CH 1995. Collins and Lyne's, Microbiological Methods. Seventh Edition. Buuterworth Heinamann. Oxford London.

Corbert EF, Wong MCM, Lin HC 1997. Periodontal Attachment Loss in Adult in Guandong RRC,J.Dent.Res.5:1176-50.

Depkes RI 2000. Standar Operating Prosedur (SOP) in Microbiology, Laboratoriun Kesehatan Departemen Kesehatan, Jakarta.

Eick S, Schmitt A, Sachse S et al., 2004. In vitro antibacterial activity of fluoroquinolones against Porphyromonas gingivalis strains. Journal of

Antimicrobial Chemotherapy.54(2):553-556.

Enersen M, Olsen I et al., 2005. Multilocus Sequence Typing of Porphyromonas gingivalis Strains from Different Geographic Origins. Institute of Oral Biology, Dental Faculty, University of Oslo, Departement of Oral Microbiology, Academic Center for Dentistry Amsterdam, The Netherlands.

Fishman Stuart L 2000. Summary of Brush Up in Wellness Symposium, Meeting Summary. J. Periodontal.71:679-682.

Gabastou JM et al., 1995. Phenotypes of resistance to antibiotics of the most frequently isolated strains in five specialized hospital centers. Multicenter study. PubMed indexed for MEDLINE, vol 43 (4), Apr, hal 320-323, http://www.ncbi.nlm.nih.gov/entrez/query.fc gi?md=Retrieve&db=PubMed&list, diakses 31 Mei 2006.

Grkovic S, Brown MH and Skurray RA 2002. Regulation of bacterial drug export systems. Microbiol. Mol. Biol., Rev 66:671 701.

Haake SK 1993. Microbiology of Dental Plaque. California Continuing Education.

Han XY and Andrade RA 2004. Brevundimonas diminuta infections and its resistance to fluoroquinolones. Section of Clinical Microbiology, The University of Texas.

Hatta M and Smits L, Henk 2007. Detection of Salmonella typhi by Nested Polymerase Chain Reaction in blood, urine and stool samples.

American J. Tropical Medicine Hygiene.Vol: 76;139-143.

Hooper DC, Wolfson JS, Bozza MA and Ng EY 2002.Genetics and regulation of outer membrane protein expression by quinolone resistance loci nfxB, nfxC, and cfxB. Antimicrob. Agents Chemother, 36:1151 1154.

Izadi Shima Mahdavi 1998. Periodontal Disease, Oral Health and Cardiovascular Disease Among Females in Sweden.J.Dental Ed.395-404.

Kasuga Y, Ishihara K, Okuda K 2000. Significance of Detectioning Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola in Periodontal Pockets.Bull Tokyo DENT. Coll. 41(3):109-117.

Morinushi T, Lopatin DE, Poperin NV et al., 2000. The Relationship Between Gingivitis and Colonization by Porphyromonas gingivitis and

Actinobacillus actinomycetemcomitans in Children. J.Periodontal.71:403-409.

Naim R 2003. Cara Kerja dan Mekanisme Resistensi Antibiotik. Harian Kompas. Kompas Online. Qarah SMD 1992.Pseudomonas aeruginosa Infections. Dermatology Online Journal. May-Jun; 18(3): 187-121.

Ronderos M, Michalowicz B, Camara R, Contreras A 2000. Bacterial and Viral Risk Markers for Juvenile Periodontitis.J.Periodontal.71.1208.




DOI: http://dx.doi.org/10.33476/jky.v17i1.192

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 YARSI Medical Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

__________________________________________________________________________________________

Copyright of YARSI Medical Journal.

Powered by OJS.

Creative Commons License

This work is licensed under a Creative Commons Attribution- NonCommercial 4.0 International License.