Studi Leptospira sp Pada Beberapa Daerah Rawan Banjir di Jakarta

Dian Widiyanti, S.Si, M.Si, PhD, Ike Irmawati Purbo Astuti

Abstract


Leptospirosis termasuk re-emerging disease dan sering menjadi wabah setelah bencana banjir. Penyakit ini disebabkan oleh bakteri Leptospira patogen yang ditansmisikan secara langsung lewat hewan terinfeksi atau tidak langsung melalui lingkungan yang terkontaminasi urin hewan tersebut. Studi mengenai Leptospira yang ada di lingkungan perairan daerah rawan banjir dilakukan untuk mengetahui penyebaran Leptospira, terutama strain patogen, sehingga dapat dilakukan antisipasi pencegahan. Sampel dikumpulkan dan diukur pHnya dari 20 penampungan air, seperti waduk, danau, sungai, selokan air, di daerah rawan banjir di Jakarta, dan dikultur pada medium Korthof modifikasi mengandung 5-fluorouracil. Pengamatan hasil kultur dilakukan dengan mikroskop lapang gelap selama satu bulan. Diferensiasi Leptospira dilakukan dengan deteksi gen flaB. Hasil menunjukkan bahwa 75% dari sampel yang diperoleh, positif Leptospira. Ph sampel air sebesar 6,6–7,9 masih sesuai untuk pertumbuhan Leptospira. Analisis dengan gen flaB menunjukkan bahwa Leptospira yang diisolasi termasuk jenis saprofit. 

Keywords


Leptospira; banjir; flaB; patogenik

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DOI: https://doi.org/10.33476/jky.v24i2.121

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