Cytotoxicity of Physic Nut (Jatropha curcas L., Euphorbiaceae)Latex by Agar-Overlay

Authors

  • Fazwishni Siregar Dentistry Study Programme, Faculty of Medicine, YARSI University, Jakarta

https://doi.org/10.33476/jky.v23i3.229

Keywords:

Agar Overlay Technique, Cytotoxicity Test, Fibroblast L929, Jatropha Curcas Latex, Toxicology

Abstract

Jatropha curcas L. latex has been used as traditional medicine to cure various infections among others are to cure toothache, apthae, as a mouth rinse to treat bleeding gums, as a hemostatic, and a wound dressing. To be used as herbal medicine, scientific investigation including toxicological studies must be conducted. The aim of these studies was to evaluate the cytotoxicity of J. curcas latex by agar overlay technique as part of toxicological study. Fibroblasts L929 were cultured in Petri dishes to confluence, and a layer of agar was then added. After staining the cells with neutral red, diluted latex (0.4-15% w/v) imbibed in filter paper were applied to the agarsurface and incubated for 24 hours. Evaluation was based on the zone of decoloration and lysis indices. Result showed that there was zone of decoloration from 2-5 mm in diameter that was all in the index 2 (less than 5 mm) from 5 index decoloration classification. Lyses cells were not observed in the decoloration zone, which could be interpreted as a coagulative necrosis. It may be concluded thatJ. curcaslatex has a moderate cytotoxic activity and necrotic coagulation in an agar-overlay technique usingfibroblast L929.

References

de Feo V 1989. Uso di planti ad azione antiinflammatoria nell’Alto Ucayall, Peru Orientale.Fitoterapia.62:481-494.

Dewi Fatma S, Widurini, Farida R, Irmaleny 2012. In vitro cytotoxicity of Jatropha curcas in epithelial and fibroblast cells. J Nat Prod. 5: 214-221.

Dos Santos RL, Pithon MM, de Oliveira MV, da Silva Mendas G, Romanos MTV, de Oliveira Ruellas AC2008. Cytotoxicity of intraoral orthodontic elastics. Braz J Oral Sci.7:1520-1525.

Goswani NK, Saharia D, Kar A 2013. Traditional uses of Jatropha curcas

Linnaeus (euphorbiaceae) as medicine by differnet communities in Northeast India. Pleione. 7: 66-72.

Heyne K 1987. Tumbuhan Berguna Indonesia, Vol II. 1st Ed. Jakarta: Translated by Litbang Kehutanan. p 1180-1182.

International Standard organization 1997. Biological Evaluation of Dental Materials. Technical Report No 7405.

Kone-Bamba D, Pelissier Y, Ozoukou ZF and Kouao D 1987. Haemostatic activity of fifteen medicinal plants of the traditional medicine of the Ivory Coast. Plant Med Phytother. 21: 122130.

Messer HH, Feigal RJ 1985. A comparison of the antibacterial and cytotoxic effects of paraphenol. J Dent Res. 64:818-821.

Nath LK, Dutta SK 1991. Extraction and purification of curcain, a protease from the latex of Jatropha curcas Linn. Indian J Pharmacol.43:111-114.

Nath LK, Dutta SK 1989. A kinetic study on curcain, a protease from the latex of Jatropha curcas Linn. Indian J Pharmaceut Sci. 51: 43-47.

Oh KT, Kim KN 2005. Ion release and cytotoxicity of stainless steel wires. Europ J Orthod. 27(6): 533-540.

Park YJ, Song YH, An JH, Song HJ, Anusavice K2013. Cytocompatibility

of pure metals and experimental binary titanium alloys for implant

materials. J Dent.41:1251-1258.

Perry LM, Metzger J 1980. Medicinal Plants of East and Southeast Asia.

Cambridge: MIT Press. p 246-247.

Prasad, DMR, Izam A, Khan MdMR 2012. Jatropha curcas: plant of medicinal benefits. J Med Plant Res. 6: 2691-2699.

Siregar F, Soerono Akbar SM, Chairul2001. Phytochemical screening and hemolytic activity of Jatropha curcas L. (Euphorbiaceae) latex. Proceeding International Seminar on Natural Product Chemistry and Utilization of Natural Resources. Depok.

Siregar F, Akbar SMS 2000. Cytotoxicity of Jatropha curcas (Euphorbiaceae) latex on fibroblast by MTT assays. Med J Indones.9: 253-257.

Siregar F, Kristiani I 2007. Mutagenicity activity of Jatropha curcas L

(Euphorbiaceae) latex. Berkala Ilmu Kedokteran. 39: 23-26.

Suwondo S1993. Improving the use of the Indonesian traditional herbal medicine for the prevention of caries and gingivitison the basis of antibacterial activity test against streptococcus mutans and clinical trial on gingivitis. Dissertation, Faculty of Dentistry, University of Padjadjaran, Bandung.

Tunc ES, Oxer L, Sari S, Cetiner S2009. Cytotoxic effects of halogen and light emitting diode cured compomers on human pulp fibroblast. Int J Peadiatric Dent.19:55-60.

Tyler VE, Brady LR, Robbers JE 1988. Pharmacognosy. 9 ed., Lea & Febiger, Philadelphia. p 67-68, 77-78, 97-98.

Velasco-Ortega E, Jos A, Camean AM, Pato-Mourelo J, Segura-Egea JJ 2010. In vitro evaluation of cytotoxicity and genotoxicity of a commercial titanium alloy for dental implantology. Mutat. Res.: Genet. Toxicol. Environ. Mutagen. doi:10.1016/j.mrgentox.2010.06.013

Windholz ML 1976. The Merck Index.#8123, #8828. Merck & Co., Darmstadt.

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Published

2017-05-02