Pengaruh antioksidan Probucol terhadap kadar Malondialdehide (MDA) dan jumlah “Circulating Endothel Cells” pada tikus putih yang menerima stressor

Abstract

The obyective of the study was to find out the effect of Probucol as an antioxidant on Malondialdehyde (MDA) level and total circulating endothelial cells of rats exposed to stressor.This study was a post test only control group design using 35 three- months old male wistar rats devided into 5 groups consisting of 7 rats each namely the control groups (Po1, Po2) and the experiment groups (P1,P2, P3). Details of the treatment to each groups ere as follows:

Group Po1 were given per oral Probucol dilution solution for 3 weeks

Group Po2 were given per oral Probucol dilution solution and stressor for 3 weeks

Group P1 were given per oral Probucol dose I (5 mg/rat) and stressor for 3 weeks

Group P2 were given per oral Probucol dose II (10 mg/rat) and stressor for 3 weeks, and

Group P3 were given per oral Probucol dose III (20 mg/rat) and stressor for 3 weeks

Malondialdehyde (MDA) level and total circulating endothelial cells were measured using “Buege and Aust (1978) and Hladovic and Rossman (1973)“ method modified by Wijayanto  (1996). Data on MDA level and total circulating endothelial cells were analyzed by using analysis of variant (Anova). The presence of significant difference was confirmed by using LSD test. Correlation between MDA level and total circulating endothelial cells was analysed by using “scarter plot” and r (rho) tests. The results showed a significant increase of MDA level of Po1 compared to Po2 (P less than 0.05). Significant decrease of MDA level (P less than 0,05) was observed in group  P1, P2 and P3 compared to that of the Po1 and Po2 control groups. The results also showed a significant increase of total circulating endothelial cells of the control group  Po1 compared to that in the control group Po2 (P less than 0,05), while significant decrease of total circulating endothelial  cells (P less than 0,05) was observed in group P1, P2 and P3 compared to that of the control group Po2. It can be concluded that stressor may increase MDA level and total circulating endothelial cells