Pengendalian vektor malaria An. maculatus menggunakan Bacillus thuringiensis H-14 galur lokal di Kecamatan Kokap, Kabupaten Kulon Progo, DIY

Authors

  • Blondine Ch.P Blondine Ch.P

https://doi.org/10.33476/jky.v13i1.1046

Keywords:

Vector control, B. thuringiensis H-14, local strain, An. maculatus

Abstract

Biological control using bioinsecticide containing active Bacillus thuringiensis H-14 local strain had been done in the laboratory of the Vector and Reservoir Control Research Unit and in  breeding ponds of Anopheles maculatus in Kokap district, Kulon Progo regency. The objectives of this study were:(1). To know the efficacy of B. thuringiensis H-14 local strain toward An. maculatus larvae in the laboratory. (2). To know the effectiveness of B. thuringiensis H-14 local strain at dosages of 1 x LC95, 5 x LC95 and 10 x LC95 toward An. maculatus larvae in the field. Bacillus thuringiensis H-14 local strain at dosages of 2.145 ppm (1 x LC95), 10.724 ppm (5 x LC95) and 21.448 ppm (10 x LC95) were applied to 8 ponds with the widths of ponds ranging from 0.08 – 0.45 m2, 0.29 – 0. 64 m2 and from 0.08 – 0.79 m2 respectively. The results showed, that dosages of B. thuringiensis H-14 local strain after 24 hours were able to kill An. maculatus at percentages of 50%, 90% and 95% respectively, while after 48 hours the dosages were 7.74 ppm (LC50), 17.06 ppm (LC90) and 21.34 ppm
(LC95) with the same ability to kill the larvae of the mosquitoes. The effectiveness of B. thuringiensis H-14 local strain dosages of 2.145 ppm
(1 x LC95) toward An. maculatus larvae could read until 50% survival of the same time (7.35 days) as that of B. thuringiensis H-14 (8.14 days) at dosages of 10.724 ppm (5 x LC95). The effectiveness to kill of B.thuringiensis H-14 local strain at a dosage of 21.448 ppm (10xLC95) toward An. maculatus larvae until 50% which survive more longer after
(16.21days) than B. thuringiensis H-14 local strain 1 x LC95 and 5 x LC95 The B.thuringiensis H-14 local strain was indeed effective for controlling mosquitoes larvae

References

Anonimus 1983. ”Vectobac 12 AS, Biological Larvicide Liquid”. Abbott Laboratories, North Chicago.

Anonimus 1993. “Bt H-14 Life Cycle, The Sequence of Events Associated with Using B. thuringiensis israelensis (Bti) for the Control of Mosquito Larvae”. Abbott Laboratories , North Chicago.

Aly C 1983. “Feeding Behavior of Aedes vexans Larvae (Diptera, Culicidae) and its Influence on the Effectiveness of Bacillus thuringiensis var israelensis”, Bull.Soc.Vector Ecol., 8(2), 94 – 100.

Aly C, Mulla MS, Schnetter W and Bo-Zhao Xu 1987. ”Floating Bait Formulations Increase Effectiveness of B. thuringiensis var. Israelensis against Anopheles larvae”, Journ.of.Am.Mosq.Contr. Assoc., 3(4),583-588.

Atmosoedjono S 1993. ”Komunikasi Pribadi Tentang Hasil Pemeriksaan Sporozoit Pada Nyamuk Anopheles dari Kecamatan Kokap”. Dalam Barodji, U. Widyastuti., T. Sularto., Mujiono dan Tri Suwarjono. 1995. Survei Jentik Anopheles dan Potensi Nyamuk yang Ditemukan dalam Penularan Malaria di Kecamatan Kokap, Kabupaten Kulon Progo, DIY, Majalah Kesehatan Masyarakat.,53, 20-23

Becker N and Margalit J 1992. “Control of Diptera with B. thuringiensis israelensis”, Training in Tropical Diseases., Jenewa 4.

Blondine Ch. P dan Widyastuti U 1991. “Isolasi Bacillus thuringiensis dari Tanah pada Pohon di Kotamadia Salatiga”, Prosiding Seminar Ilmiah dan Kongres Nasional Biologi X, Bogor.

Blondine Ch.P, Widyastuti U, Widiarti, Sukarno dan Subiantoro 1999. “Uji Serologi Isolat Bacillus thuringiensis dan Patogenisitasnya Terhadap Jentik Nyamuk Vektor”, Bull. Pen. Kes., 26 (2&3), 91-98.

Chilcott CN and Pillai JS 1985. “The Use of Coconut Wastes for the Production of Bacillus thuringiensis var israelensis”, Micren Journal.,1,327-332.

Devidas P 1992. “Bt mode action: Approaches. Dalam Sem. Proc. Global Management of Insecticide Resistance in the 90’s. September”, 15-17.

Finney DJ 1971. “Probit Analysis”, 3rd, ed.,Cambridge Univ.Press.London .

Jaquet F, Hunter R and Luthy P 1987. “Specificity of Bacillus thuringiensis deltaendotoxin”, Appl.Environ.Micro., 53(3),500-504.

Komite Review 1998. “Review Comprehensive untuk Supresi Foci Malaria di Kabupaten Kulon Progo, Purworejo”.

Lee HL, Pe. TH and Cheong WH 1986. “Laboratory Evaluation of the Persistence of Bacillus thuringiensis var israelensis Against Aedes aegypti Larvae”, Mosq. Born.Dis.Bull., 2(3):61-66.

Lee HL 1988. “Isolation and Evaluation of Two Isolates of Bacillus thuringiesnsis for the Control of Mosquitoes of Public Health Importance in Malaysia”, Mosq. Born.Dis.Bull., 5(3-4), 39-47.

Mian LC and Mulla MS 1983. “Factor Influencing Activity of the Microbial Agent B.sphaericus Against Mosquito Larvae”, Bull.Soc.Vector Ecol.,8(2),128-34.

Mulla MS, Norland RL, Fanara DM, Darwazeh AM and Mc Kean DW 1971. “Control of Chironomid Nudges in Recreational Lakes”. J.Econ.Entomol., 71,774-777.

Mulla MS, Darwazeh HA, Davidson EW and Dulmage HT 1984. ”Efficacy and Persistence of the Microbial Agent B. sphaericus Against Mosquito Larvae in Organically Enriched Habitats”, Mosq.News.,44, 166-173.

Mulla MS, Darwazeh HA and Aly C 1986. “Laboratory and Field Studies on New Formulations of Two Microbial Control Agents Against Mosquitoes”.Bull.Soc.Vector Ecol., 11(2),255-63.

Nguyen TTH, Su T and Mulla MS 1999. “Mosquito Control and Bacterial Flora in Water Enriched with Organic Matter and Treated with Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus Formulations”, Journal of Vector Ecology., 24(2),138-153.

Ramoska WA and Hopkins TL 1981. “Effects of Mosquito Larval Feeding Behavior on B. sphaericus Efficacy . J. Invert. Pathol., 37, 269-72

Ravoahangimalala O, Thiery I and Sinegre G 1994. “Rice Field Efficacy of Deltamethrin and Bacillus thuringiensis israelensis Formulations on Anopheles gambiae S.S. in the Anjiro Region of Madagascar”, Bull.Soc.Vector Ecol., 19(2),169-174.

Sundararaman S, Soroto RM and Siran M 1957. ”Vectors of Malaria in Mid Java”, Indian J .Mlariology., 11,321-338.

WHO 1979. “Data Sheet on the Biological Control Agent. Bacillus thuringiensis serotype H-14”, WHO/VBC/79.750. 1-13.

WHO 1989. “Informal Consultation of Bacterial Formulations for Cost-Effective Vector Control in Endemic Area”, WHO/VBC/89.979.

WHO 1999. “ Guideline Specifications for Bacterial Larvicides for Public Health Use”, WHO/CDS/CPC/WHOPES/ 99.2.

Widyastuti U, Widiarti, dan Blondine Ch.P 1995. “Uji Coba Bacillus thuringiensis H-14 Terhadap Jentik Nyamuk Anopheles barbirostris di Laboratorium dan Lapangan”, Bull.Pen.Kes., 23(1),39-45.

Widyastuti U, Blondine Ch. P dan Mujiyono 1999. “Uji Coba Vectobac G (B. thuringiensis H-14) Terhadap Jentik Anopheles spp di Sawah Desa Bawonifaoso, Kecamatan Teluk Dalam, Kabupaten Nias”, Majalah Kesehatan Masyarakat Departemen Kesehatan R.I., 61,33-37.

Downloads

Published

2019-11-20

Issue

Section

Research Articles