Evaluasi Kemampuan Proliferasi, Diferensiasi dan Viabilitas Sel Punca Asal Pulpa Gigi Manusia Pasca Vitrifikasi

Restu Syamsul Hadi, Indra Kusuma

Abstract


Sejumlah keterbatasan sumber sel pulpa gigi manusia diantaranya harus dari pulpa gigi sehat dengan jumlah sangat terbatas. Teknologi penyimpanan sel dan pengembangan metode vitrifikasi pada DPSC dan MSC sangat bermanfaat untuk keperluan terapi. Penelitian ini  bertujuan mengkaji pertumbuhan, proliferasi dan diferensiasi serta pengaruh simpan beku metode vitrifikasi terhadap sel punca asal pulpa gigi. Sampel yang digunakan adalah pulpa gigi yang diisolasi. Untuk mengukur proliferasinya digunakan Cell Proliferation Reagent WST-1 Absorbansi diukur menggunakan mikroplate ELISA Zenyth pada panjang gelombang 450 nm. Untuk proses simpan beku dengan metode vitrifikasi sel MSC dipanen dan di-cryopreservasi dengan dua langkah yaitu diberikan dengan Equilibration (EQ) dan vitrifikasi solutions (VS). Untuk diferensiasi sel adipogenik digunakan StemPro Adipogenesis dan diuji dengan pewarnaan Oil Red O. DPSC berhasil diisolasi dan tumbuh dengan baik pada medium alfa MEM dengan serum 10%. Kemampuan proliferasi sel DPSC pada inkubasi 24 jam lebih tinggi secara signifikan dibandingkan inkubasi 18 jam. DPSC dapat terdiferensiasi menjadi neural like cells dan pada sel MSC dengan pemberian differentiation Kit StemPro Adipogenesis, diferensiasi MSC ke arah sel adipogenik setelah pewarnaan menggunakan Oil Red O. Teknik vitrifikasi sebagai metode simpan beku pada MSC menunjukan viabilitas yang tinggi (> 80%) dan sel mampu berproliferasi dan berdiferensiasi dengan baik.


Keywords


dental pulp, diferensiasi, viabilitas, vitrifikasi

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DOI: https://doi.org/10.33476/mkp.v11i1.951

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