Trial of Neem Oil (Azadirachta indica) as Basic Compound of Electric Liquid Vaporizer against Aedes aegypti Mortality

Authors

  • Ajib Diptyanusa Department of Parasitology, Faculty of Medicine Universitas Gadjah Mada
  • Tri Baskoro Tunggul Satoto Department of Parasitology, Faculty of Medicine Universitas Gadjah Mada
  • Tridjoko Hadianto Department of Parasitology, Faculty of Medicine Universitas Gadjah Mada

https://doi.org/10.33476/jky.v25i1.296

Keywords:

Aedes aegypti, electric, vaporizer, neem, Azadirachta indica

Abstract

Dengue Hemorrhagic Fever (DHF), commonly caused by Aedes aegypti mosquito bites, has been one of the world’s major concern for progressively increasing incidence. To prevent further increase in DHF incidence, an effective yet safe vector control method is needed. One of the most common method of vector control in Indonesia is using electric liquid vaporizer. Basic compounds which are less toxic to humans and less resistance-producing to mosquitoes are preferred, without neglecting its ability in killing mosquitoes. Neem trees (Azadirachta indica) could be easily found in many areas in Indonesia, mainly functioning as shading trees. Leaves and seed of neem tree may contain active compound used as natural insecticides, azadirachtin. The research aims to identify killing effect of neem oil as basic compound of electric liquid vaporizer against Aedes aegypti. Research subjects were 275 Aedes aegypti mosquitoes, aged 2-5 days. Subjects were divided into 3 groups: group A (negative control), group B (100% neem oil), and group C (0.001% d-allethrin as positive control). Knockdown time (KT1, KT50, KT95, KT100) and 24-hours mortality were observed. Test replications were done 3 times. The results showed that pure neem oil (Azadirachta indica) has no direct killing effect against Aedes aegypti. Further research is encouraged regarding identification of adulticide characteristics of azadirachtin and other active compounds of neem oil, such as nimbidin and nimbin.

Author Biography

Ajib Diptyanusa, Department of Parasitology, Faculty of Medicine Universitas Gadjah Mada

Junior Lecturer at Department of Parasitology, Faculty of Medicine Universitas Gadjah Mada

References

Abbey, T. K., and J. W. Essiah. 1990. Physics for senior secondary schools. Macmillan Education, Ltd., London.

Alouani, A., N. Rehimi, and N. Soltani. 2009. Larvicidal Activity of a Neem Tree Extract (Azadirachtin) Against Mosquito Larvae in the Republic of Algeria. Jordan J. Biol. Sci. 2: 15–22.

Aremu, O. I., M. N. Femi-Oyewo, and K. O. K. Popoola. 2009. Repellent Action of Neem (Azadirachta indica) Seed Oil Cream against Anopheles Gambiae Mosquitoes. African Res. Rev. 3: 12–22.

Asogwa, E. U., T. C. N. Ndubuaku, J. A. Ugwu, and O. O. Awe. 2010. Prospects of botanical pesticides from neem , Azadirachta indica for routine protection of cocoa farms against the brown cocoa mirid – Sahlbergella singularis in Nigeria. J. Med. Plant. Res. 4: 1–6.

Bhatt, S., P. W. Gething, O. J. Brady, J. P. Messina, A. W. Farlow, C. L. Moyes, J. M. Drake, J. S. Brownstein, A. G. Hoen, M. F. Myers, D. B. George, T. Jaenisch, and G. R. William. 2013. The global distribution and burden of dengue. Nature. 496: 504–507.

Biswas, K., I. Chattopadhyay, and R. K. Banerjee. 2002. Biological activities and medicinal properties of neem (Azadirachta indica). Curr. Sci. 82: 1336–1345.

Bond, E.J., and H. A. U. Monro. 1984. Manual of fumigation for insect control. Food and Agriculture Organization of the United Nations, Rome.

Brady, O. J., P. W. Gething, S. Bhatt, J. P. Messina, J. S. Brownstein, A. G. Hoen, C. L. Moyes, A. W. Farlow, T. W. Scott, and S. I. Hay. 2012. Refining the Global Spatial Limits of Dengue Virus Transmission by Evidence-Based Consensus. PLoS Negl Trop Dis. 6: 1–15.

Caballero-anthony, M., A. D. B. Cook, G. Gayle, H. Amul, and A. Sharma. 2015. Health governance and dengue in Southeast Asia. Singapore.

(CDC) Center for Disease Control and Prevention. 2010. Dengue. http://www.cdc.gov/dengue/ accessed March 28, 2014.

Costero, A., J. D. Edman, G. G. Clark, P. Kittayapong, and T.W. Scott. 1999. Survival of starved Aedes aegypti (Diptera: Culicidae) in Puerto Rico and Thailand. Journal of Medical Entomology. 36(3): 272-6.

Federer, W. 1991. Statistics and society: data collection and interpretation, 2nd ed. Marcel Dekker, New York.

Guomin, J., Y. Xiaolin, and C. Rongchang. 2003. The handbook of insecticide formulations and its technologies for household and public health uses. Jin Tai Printing Ltd., Hong Kong.

Kesetyaningsih, T. W. 2008. Khasiat Obat Nyamuk Bakar Berbahan Aktif Pyrethroid terhadap Culex quinquefasciatus pada Berbagai Kondisi Ruangan. Mutiara Medika. 8(2): 67–76.

Kiplang’at, K. P., and R. W. Mwangi. 2013. Repellent Activities of Ocimum basilicum, Azadirachta indica and Eucalyptus citriodora Extracts on Rabbit Skin against Aedes aegypti. J. Entomol. Zool. Stud. 1: 84–91.

Kumar, R. 1986. Insect pest control with special reference to African agriculture, 1st ed. The Camelot Press Ltd., Southcampton, Great Britain.

Lloyd, L. S. 2003. Best practices for dengue prevention and control in the Americas. Office of Health, Infectious Diseases and Nutrition Bureau for Global Health, U.S. Agency for International Development, Washington, DC.

Maia, M. F., and S. J. Moore. 2011. Plant-based insect repellents: a review of their efficacy, development and testing. Malar. J. 10: 1–14.

Mandal, S. 2011. Repellent activity of Eucalyptus and Azadirachta indica seed oil against the filarial mosquito Culex quinquefasciatus Say (Diptera: Culicidae) in India. Asian Pac. J. Trop. Biomed. 109–112.

Mardisiswojo, S. and H. Rajakmangunsudarso. 1985. Cabe puyang warisan nenek moyang. Balai Pustaka, Jakarta.

Ndione, R. D., O. Faye, M. Ndiaye, A. Dieye, and J. Marie. 2007. Toxic effects of neem products (Azadirachta indica A . Juss) on Aedes aegypti Linnaeus 1762 larvae. Afr. J. Biotechnol. 6: 2846–2854.

Quarles, W. 1994. Neem tree pesticides product ornamental plants. The IPM Practitioner. 16(10): 1–13.

Sharma, V. P., M. A. Ansari, and R. K. Razdan. 1993. Mosquito Repellent Action of Neem (Azadirachta indica) Oil. J. Am. Mosq. Control Assoc. 9: 359–360.

Shivakumar, M. S. 2011. Comparative efficacy of Azadirachtin on the larval population of Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti (Diptera: Culicidae) in Gujarat, India. Int. J. Pharma Bio Sci. 2: 41–47.

Soedarto. 1990. Entomologi kedokteran. EGC, Jakarta, Indonesia.

Sugito, R. 1989. Aspek etmologi demam berdarah, pp. 37–43 In B. Haryanto, R. H. Sri, W. Suharyono, and I. M. Djaya, Berbagai aspek DBD dan penanggulangannya. Annual Report, Depok, Indonesia.

Supargiyono. 1988. Pengaruh Asap Obat Nyamuk Bakar Terhadap Lama Hidup, Kemampuan Bertelur, Daya Tetas Telur, dan Daya Tetas Larva Aedes aegypti. B.I.Ked. Fakultas Kedokteran UGM. 20: 61–68.

Sutanto, B.A. and Purnomo. 1990. Pemakaian repelen komersial yang mengandung Toluamide terhadap Culex quinquefasciatus di Jakarta. Medika. 16: 38-42.

Valecha, N., M.A. Ansaari, S. Prabhu and R. K. Razdan. 1996. Preliminary evaluation of safety aspects of neem oil in kerosene lamp. Indian J. Malariol. 33: 139–143.

(WHO) World Health Organization. 2015. Dengue and Severe Dengue. http://www.who.int/mediacentre/factsheets/fs117/en/ accessed February 11, 2016.

World Health Organization. 2011. Comprehensive Guidelines for Prevention and Control of Dengue and Dengue Haemorrhagic Fever, Revised an. ed. World Health Organization, Regional Office for South-East Asia, New Delhi.

Yogyakarta District Health Office. 2013. Yogyakarta health profile year 2013. Yogyakarta District Health Office, Yogyakarta Special Region, Indonesia.

Downloads

Published

2017-07-04

Issue

Section

Research Articles