Pola mRNA Hypoxia Inducible Factor-1a (HIF-1a) dan Exkspresi Protein HIF-1a Ginjal Tikus pada Hipoksia Sistemik Kronik
Authors
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Yulia Suciati
Department of Biochemistry
Faculty of Medicine
YARSI University, Jakarta
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Ani Retno Prijanti
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Mohamad Sadikin
Keywords:
Hipoksia, ekspresi gen HIF-1?, Western BlottAbstract
Ekspresi protein HIF-1a pada organ ginjal dari tikus yang mengalamikondisi hipoksia secara sistemik menggunakan Hypoxic Chamber dengankadar O2 8% dan Nitrogen 92% dan kelompok kontrol pada kondisinormoksia. Pola mRNA HIF-1a dilihat berdasarkan hasil RT-PCR denganmembandingkan rasio kelompok normoksia dan kelompok hipoksia yangmenunjukkan terdapat peningkatan ekspresi mRNA HIF-1a sejalan denganlamanya hipoksia yang mencapai puncak pada kelompok hipoksia 3 hari danmulai mengalami penurunan pada kelompok 7 hari. Ekspresi protein HIF-1a dilakukan dengan metode Western Blott yang memperlihatkan terdapatnyapeningkatan ekspresi protein HIF-1a yang mulai mengalami penurunan padakelompok hipoksia 14 hari.enelitian ini bertujuan untuk mengetahui bagaimana pola mRNA HIF-1a dan ekspresi protein HIF-1a pada organ ginjal dari tikus yang mengalamikondisi hipoksia secara sistemik menggunakan Hypoxic Chamber dengankadar O2 8% dan Nitrogen 92% dan kelompok kontrol pada kondisinormoksia. Pola mRNA HIF-1a dilihat berdasarkan hasil RT-PCR denganmembandingkan rasio kelompok normoksia dan kelompok hipoksia yangmenunjukkan terdapat peningkatan ekspresi mRNA HIF-1a sejalan denganlamanya hipoksia yang mencapai puncak pada kelompok hipoksia 3 hari danmulai mengalami penurunan pada kelompok 7 hari. Ekspresi protein HIF-1a dilakukan dengan metode Western Blott yang memperlihatkan terdapatnyapeningkatan ekspresi protein HIF-1a yang mulai mengalami penurunan padakelompok hipoksia 14 hari.References
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